different methods for diagnosis of brucella and legionella spp. in various samples

community-acquired pneumonia and brucellosis are of the main agents causing mortality in the world. the most important limitations for the identification of these agents include their high resistance against environmental conditions, impossibility of rapid and on time diagnosis, low level of infective dose, and lack of existence of vaccine against many types of them. hence, on time action and diagnosis of agent of infectious diseases has been regarded as a solution for preventing incidence of this type of disease. because of the danger of brucella and legionella bacteria, studying isolation methods is one of the most important measures regarding detection of agent of this type of attacks. the present study is a review study, which is conducted using existing studies and also using library method in order to investigate the detection of two mentioned bacteria in various samples.